Rabbit Anti-Myc tag agarose Beads were used to immunoprecipitate 2 x Myc tag-mouse IgE-His tag from Expi293F system.
During elution with Pepide elution buffer, the enriched Myc fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: 2 x Myc tag-mouse IgE-His tag protein.
Rabbit Anti-Myc tag agarose beads
Rabbit Anti-Myc tag agarose beads
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IP, Protein purification, Co-IP, ChIP |
| Purification | Protein A |
| Conjugation | Agarose beads |
| Physical Appearance | Liquid |
| Storage Buffer | 50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion. |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
The c-Myc is a polypeptide protein tag derived from the c-myc gene product, which can be added to the protein by recombinant DNA technology. It contains 10 amino acids (EQKLISEEDL) with a molecular weight of 1.2 KDa and can be fused to the N-terminus or C-terminus of the target protein for purification and detection. The Myc tag is widely used in various expression systems, including bacteria, yeast, insects and mammalian cells, to detect the expression of fusion proteins. It can be used for affinity chromatography and purified by coupling the Myc-tagged antibody to divinylsulfone-activated agarose.
Picture
Picture
SDS-PAGE
Rabbit Anti-Myc tag agarose Beads were used to immunoprecipitate 2 x Myc tag-mouse IgE-His tag from Expi293F system.
During elution with Pepide elution buffer, the enriched Myc fusion protein are released from the agarose beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: 2xMyc tag-mouse IgE-His tag protein.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with an MYC-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Rabbit Anti-Myc tag agarose beads, H3 beads and Rabbit lgG lsotype Control agarose beads for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of RPL30, MYOD1 and SAT-α in Rabbit Anti-Myc tag agarose beads-immunoprecipitated sample.
