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Rabbit Anti-Mouse/Rat IgG (Light chain specific) (HRP Conjugate)

Rabbit Anti-Mouse/Rat IgG (Light chain specific) (HRP Conjugate)

Catalog Number: S0B4078 Application: ELISA, WB Reactivity: Ms, Rt Conjugation: HRP Brand: Starter
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Regular price $270 USD
Regular price Sale price $270 USD
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Product Details

Product Specification


Host Rabbit
Antigen Mouse/Rat IgG
Immunogen Recombinant Protein
Clone Number S-2695-12
Antibody Type Recombinant mAb
Isotype IgG
Application ELISA, WB
Reactivity Ms, Rt
Purification Protein A
Concentration 1 mg/ml
Conjugation HRP
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Background

Based on ELISA, this antibody specifically reacts with mouse/rat IgG light chain.

Picture

Western Blot

WB result of mouse IgG Recombinant Rabbit mAb
Primary antibody: mouse IgG Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 μg
Lane 2: mouse IgG protein lysate 1 μg
Lane 3: mouse IgG protein lysate 100 ng
Lane 4: mouse IgG protein lysate 10 ng
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa

ELISA

ELISA analysis of Rabbit Anti-Mouse/Rat IgG (Light chain specific) (HRP Conjugate)(S0B4078)
Coating mouse IgG, human IgG, Rat IgG, Rabbit IgG: 1μg/mL
From 2.5μg/ml, 4-fold diluent, 6 points