Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse Ly6G antibody at 1/50 (1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ antibody separately.
Rabbit Anti-Mouse Ly6G Antibody (S-2495-6)
Rabbit Anti-Mouse Ly6G Antibody (S-2495-6)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Ly6G |
| Synonyms | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-2495-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:50 | Ms |
Background
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
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