Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized C57BL/6 mouse splenocytes, Stimulated 6 hours with Stimulation Cocktail (Right panel) or untreated (Left panel) labelling Mouse IL-2 antibody at 1/2000 dilution (0.01 μg). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ antibody separately.
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | IL-2 |
Synonyms | Interleukin-2; T-cell growth factor (TCGF); Il-2; Il2 |
Immunogen | Recombinant Protein |
Location | Secreted |
Accession | P04351 |
Clone Number | S-2117-6 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | ICFCM |
Reactivity | Ms |
Purification | Protein A |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS pH7.4 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
ICFCM | 1:2000 | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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