Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling ILT4 (CD85d) antibody at 1/50 (1 μg) dilution (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD14 - Alexa Fluor® 647 separately. Gated on total viable cells.
Rabbit Anti-Human ILT4 (CD85d) Antibody (S-281-50)
Rabbit Anti-Human ILT4 (CD85d) Antibody (S-281-50)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ILT4 (CD85d) |
| Synonyms | Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4 (ILT-4), Monocyte/macrophage immunoglobulin-like receptor 10 (MIR-10), LILRB2, LIR2, MIR10 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q8N423 |
| Clone Number | S-281-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:50 | null |
Picture
Picture
FC
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / THP-1 (Human monocytic leukemia monocyte, Right) labelling ILT4 (CD85d) antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG DyLight® 488 was used as the secondary antibody.
Negative control: Jurkat
