Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is used to immunoprecipitate His-tagged fusion proteins from HEK293T cell lysates. During elution with Acidic elution buffer, the enriched His-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: His-tagged fusion protein
Lane 4: Rabbit IgG Isotype Control Magnetic Agarose
Rabbit Anti-His Tag (C-terminal) Magnetic Agarose
Rabbit Anti-His Tag (C-terminal) Magnetic Agarose
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IP, Protein purification, Co-IP |
| Reactivity | Species Independent |
| Purification | Protein A |
| Conjugation | Magnetic Agarose |
| Physical Appearance | Liquid |
| Storage Buffer | 20% slurry in PBS pH7.4, 0.05% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant His tag (C-terminal) fusion proteins in E. coli, insect cells and mammalian cells. The recombinant His tag (C-terminal) fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-His Tag (C-terminal) Magnetic Agarose. It is the excellent choice for high performance purifications.
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Picture
SDS-PAGE
Rabbit Anti-His Tag (C-terminal) Magnetic Agarose is used to immunoprecipitate His-tagged fusion proteins from HEK293T cell lysates. During elution with 2x SDS-sample buffer, the enriched His-tagged fusion proteins and antibodies are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: His-tagged fusion protein
