Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with an HA-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Rabbit Anti-HA Tag Magnetic Agarose, H3 beads and Rabbit IgG Isotype Control Magnetic Agarose for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, MYOD1 and SAT-α in
Rabbit Anti-HA Tag Magnetic Agarose-immunoprecipitated
sample.
Rabbit Anti-HA Tag Magnetic Agarose
Rabbit Anti-HA Tag Magnetic Agarose
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HA Tag |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IP, Protein purification, Co-IP, ChIP |
| Reactivity | Species Independent |
| Purification | Protein A |
| Conjugation | Magnetic Agarose |
| Physical Appearance | Liquid |
| Storage Buffer | 20% slurry in PBS pH7.4, 0.05% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-HA Tag Magnetic Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant HA tag fusion proteins in E. coli, insect cells and mammalian cells. The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Tag Magnetic Agarose. It is the excellent choice for high performance purifications.
Picture
Picture
SDS-PAGE
ChIP
