Rabbit Anti-GST Tag Magnetic Agarose is used to immunoprecipitate GST-tagged fusion proteins from Escherichia coli lysate supernatant. During elution with Acidic elution buffer, the enriched GST-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: GST-tagged fusion protein
Lane 4: Rabbit IgG Isotype Control Magnetic Agarose
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | GST Tag |
Immunogen | Recombinant Protein |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | IP, Protein purification, Co-IP |
Reactivity | Species Independent |
Conjugation | Magnetic Agarose |
Physical Appearance | Liquid |
Storage Buffer | 20% slurry in PBS pH7.4, 0.05% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-GST Tag Magnetic Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-GST Tag Magnetic Agarose. It is the excellent choice for high performance purifications.
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SDS-PAGE
