Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Acidic elution buffer, the enriched FLAG-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion protein
Lane 4: Rabbit IgG Isotype Control Magnetic Agarose
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LAG (DYKDDDDK) Tag |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IP, Protein purification, Co-IP |
| Purification | Protein A |
| Conjugation | Magnetic Agarose |
| Physical Appearance | Liquid |
| Storage Buffer | 20% slurry in PBS pH7.4, 0.05% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is an anti-FLAG Rabbit Recombinant monoclonal antibody covalently conjugated to magnetic agarose beads. It can be widely used for immunoprecipitation or purification of fusion proteins or their protein complexes with Flag tags.
Picture
Picture
SDS-PAGE
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with Peptide elution buffer, the enriched FLAG-tagged fusion proteins are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion protein
Rabbit Anti-FLAG (DYKDDDDK) Tag Magnetic Agarose is used to immunoprecipitate FLAG-tagged fusion proteins from HEK293T cell lysates. During elution with 2x SDS-sample buffer, the enriched FLAG-tagged fusion proteins and antibodies are released from the magnetic agarose.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: FLAG-tagged fusion protein
