Rab3D Recombinant Rabbit mAb (S-4406-22)

Catalog Number: S0B60121 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	Rab3D
Synonyms	Ras-related protein Rab-3D; GOV; RAB16; RAB3D
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	O95716
Clone Number	S-4406-22
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	TT, HT-29, HL-60
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:2000	Hu

Background

Rab3D is a member of the small GTPase Rab family, belonging to the Rab3 subfamily, and is primarily localized on the membrane of secretory vesicles in secretory cells, playing a critical role in regulating vesicle trafficking and exocytosis. Through interactions with upstream regulators such as GEFs and GAPs, this protein cycles between the GTP-bound active state and the GDP-bound inactive state, thereby precisely controlling vesicle maturation, mobilization, docking, and fusion with target membranes. Rab3D is expressed in various tissues and is particularly abundant in pancreatic acinar cells and adipocytes, where its function is associated with the regulation of insulin-like growth factors and metabolic balance; it also participates in regulating neurotransmitter release, gastric acid secretion, and salivary secretion, making it an indispensable regulator of vesicle trafficking in exocrine and endocrine cells. Dysfunction or genetic deficiency of Rab3D is associated with multiple diseases; for example, Rab3D knockout mice exhibit increased bone mass and accelerated bone formation phenotypes, while downregulation of Rab3D expression in humans may be closely related to osteoporosis, obesity, and certain metabolic diseases, positioning it as an important molecular target for studying vesicle trafficking mechanisms and related metabolic disorders.

Picture

Western Blot

WB result of Rab3D Recombinant Rabbit mAb
Primary antibody: Rab3D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: TT whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Rab3D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.