WB result of PXR Recombinant Rabbit mAb
Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
PXR Recombinant Rabbit mAb (S-2398-43)
PXR Recombinant Rabbit mAb (S-2398-43)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PXR |
| Synonyms | Nuclear receptor subfamily 1 group I member 2; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; Pregnane X receptor; Steroid and xenobiotic receptor (SXR); NR1I2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O75469 |
| Clone Number | S-2398-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, THP-1, mouse liver, rat liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ChIP | 1:20-1:50 | Hu |
Background
PXR protein (Pregnane X Receptor) is a nuclear receptor that plays a crucial role in the regulation of drug metabolism and detoxification processes in the body. It is primarily expressed in tissues such as the liver and intestines. When activated by various endogenous and exogenous compounds, including certain drugs, PXR can induce the expression of numerous target genes. These target genes are involved in the production of enzymes like cytochrome P450, which are key for the biotransformation of drugs and other xenobiotics. This helps the body to effectively metabolize and eliminate potentially harmful substances. PXR's activity is important for maintaining homeostasis and ensuring the proper functioning of the body's detoxification systems.
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Picture
Western Blot
WB result of PXR Recombinant Rabbit mAb
Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
WB result of PXR Recombinant Rabbit mAb
Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HepG2 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used PXR Recombinant Rabbit mAb (S-2398-43) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of HMGN4, HES7 and SAT-α in PXR Recombinant Rabbit
mAb (S-2398-43)-immunoprecipitated sample.
