WB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Lane 7: SW480 whole cell lysate 20 µg
Lane 8: TF-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDa
PTP1B Rabbit Polyclonal Antibody
PTP1B Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PTP1B |
| Synonyms | Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B (PTP-1B); PTP1B; PTPN1 |
| Immunogen | Recombinant Protein |
| Location | Endoplasmic reticulum membrane |
| Accession | P18031 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HCT 116, HeLa, HepG2, A549, MCF7, Jurkat, SW480, TF-1, NIH/3T3, RAW264.7, mouse brain, mouse heart, mouse spleen, PC-12, rat brain, rat heart, rat spleen |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Protein tyrosine phosphatase 1B (PTP1B), encoded by the PTPN1 gene, is a 50 kDa non-receptor intracellular tyrosine phosphatase anchored to the cytoplasmic face of the endoplasmic reticulum that acts as a key negative regulator of both the insulin and leptin signaling cascades by dephosphorylating the insulin receptor, IRS-1, JAK2, and leptin receptor, thereby dampening downstream PI3K/AKT and STAT3 signaling; PTP1B-null mice are lean, insulin-hypersensitive, and resistant to diet-induced obesity, while elevated PTP1B expression is associated with insulin resistance, type 2 diabetes, obesity, and several cancers (especially breast and ovarian cancer), and selective PTP1B inhibitors are actively explored as therapeutics for metabolic and oncologic disorders.
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Western Blot
WB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Lane 5: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDa
WB result of PTP1B Rabbit pAb
Primary antibody: PTP1B Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat heart lysate 20 µg
Lane 4: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 35-55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PTP1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
