PTBP1 Rabbit Polyclonal Antibody

Catalog Number: S0B60037 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	PTBP1
Synonyms	Polypyrimidine tract-binding protein 1; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I (hnRNP I); PTB
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P26599
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	Jurkat, SW480, HT-29, BxPC-3, HepG2, HEK-293, HeLa, NIH/3T3, C6, COS-7
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
IHC-P	1:250-1:1000	Hu, Ms, Rt

Background

PTBP1 (Polypyrimidine Tract-Binding Protein 1, also known as hnRNP I) is a widely expressed RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein family, playing critical regulatory roles in various RNA metabolic processes including pre-mRNA splicing, polyadenylation, mRNA stability, and translational regulation. The core structural feature of this protein is its four consecutive RNA recognition motifs (RRM1-4), among which RRM1 and RRM2 cooperatively bind to pyrimidine-rich RNA sequences (particularly polypyrimidine tracts) in an atypical manner, while RRM3 and RRM4 participate in auxiliary binding and protein-protein interactions. As a key regulator of alternative splicing, PTBP1 binds to polypyrimidine tracts flanking specific exons and inhibits spliceosome recognition of those exons through steric hindrance, thereby promoting exon skipping or inclusion. The most classic example occurs during neuronal differentiation, where the decline of PTBP1 leads to global switches in the splicing patterns of its target genes (such as NRCAM and REST), subsequently promoting neuronal maturation. In terms of subcellular localization, PTBP1 typically shuttles between the nucleus and cytoplasm, and its function exhibits high cell-type and developmental-stage specificity. Clinical studies have shown that PTBP1 is abnormally overexpressed in various malignant tumors (such as glioblastoma, hepatocellular carcinoma, pancreatic cancer, and breast cancer). By regulating the splicing of epithelial-mesenchymal transition-related genes, enhancing glycolytic metabolism, and inhibiting apoptosis, it promotes tumor cell proliferation, invasion, metastasis, and chemoresistance, making it an important candidate molecule for cancer diagnostic and prognostic markers as well as targeted therapy. Furthermore, dysregulation of PTBP1 is also closely associated with spinal muscular atrophy and certain viral infection processes, highlighting the central role of this protein in both physiological and pathological RNA processing networks.

Picture

Western Blot

WB result of PTBP1 Rabbit pAb
Primary antibody: PTBP1 Rabbit pAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SW480 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: BxPC-3 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: HEK-293 whole cell lysate 20 µg
Lane 7: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 59 kDa

WB result of PTBP1 Rabbit pAb
Primary antibody: PTBP1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 59 kDa

WB result of PTBP1 Rabbit pAb
Primary antibody: PTBP1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 59 kDa

WB result of PTBP1 Rabbit pAb
Primary antibody: PTBP1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 59 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-PTBP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PTBP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PTBP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PTBP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-PTBP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.