WB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: RPMI-8226 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDa
PSMB5 Rabbit Polyclonal Antibody
PSMB5 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PSMB5 |
| Synonyms | Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; LMPX; MB1; X |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P28074 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | K562, RPMI-8226, MCF7, T47D, Neuro-2a, mouse liver, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 | Hu, Ms, Mk |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
PSMB5, also known as proteasome subunit beta type-5 or the X subunit of the 20S core particle, is a critical catalytic component of the 26S proteasome complex responsible for intracellular protein degradation via the ubiquitin-proteasome pathway. As one of the three distinct beta subunits possessing proteolytic activity within the 20S core, PSMB5 specifically exhibits chymotrypsin-like activity, cleaving peptide bonds adjacent to hydrophobic amino acid residues, which is essential for maintaining cellular homeostasis by removing damaged, misfolded, or regulatory proteins. This subunit plays a pivotal role in antigen processing for MHC class I presentation, thereby influencing immune surveillance, and serves as the primary molecular target for bortezomib and other proteasome inhibitor drugs used clinically to treat multiple myeloma and mantle cell lymphoma; furthermore, under inflammatory conditions induced by interferon-gamma, PSMB5 can be replaced by its inducible homolog LMP7 (PSMB8) to form the immunoproteasome, which alters cleavage specificity to optimize the generation of antigenic peptides for immune recognition.
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Western Blot
WB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDa
WB result of PSMB5 Rabbit pAb
Primary antibody: PSMB5 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortx. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PSMB5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
