WB result of PSD-95 Recombinant Rabbit mAb
Primary antibody: PSD-95 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 80 kDa
Observed MW: 70~110 kDa
PSD-95 Recombinant Rabbit mAb (S-1518-4)
PSD-95 Recombinant Rabbit mAb (S-1518-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PSD-95 |
| Synonyms | Disks large homolog 4; Postsynaptic density protein 95; Synapse-associated protein 90 (SAP-90; SAP90); Dlg4; Dlgh4；PSD95 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cell membrane |
| Accession | Q62108 |
| Clone Number | S-1518-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse cerebellum, mouse brain, rat cerebellum, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| IF | 1:500 | Hu |
Background
PSD-95 (Postsynaptic Density Protein-95) is a protein highly enriched in the postsynaptic density of excitatory synapses in the central nervous system. It is a major synaptic scaffolding protein that contains three PDZ domains, one SH3 domain, and a GUK domain. These domains enable PSD-95 to interact with a variety of proteins, including NMDA and AMPA receptors, thereby playing a crucial role in synaptic plasticity and signal transduction. PSD-95 is involved in regulating the maturation and function of synapses, influencing long-term potentiation (LTP) and long-term depression (LTD). It modulates the stability and function of receptors and ion channels at the postsynaptic membrane through interactions with them. Abnormalities in PSD-95 function are associated with various neuropsychiatric disorders, including autism, schizophrenia, and intellectual disability. Mutations in the gene encoding PSD-95 in these disorders may lead to synaptic dysfunction.
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Western Blot
WB result of PSD-95 Recombinant Rabbit mAb
Primary antibody: PSD-95 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 81 kDa
Observed MW: 70~110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human retina. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
