WB result of PRTN3 Recombinant Rabbit mAb
Primary antibody: PRTN3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: GES-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HL-60 whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Negative control: GES-1 whole cell lysate, HeLa whole cell lysate, Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
PRTN3 Recombinant Rabbit mAb (S-4942-65)
PRTN3 Recombinant Rabbit mAb (S-4942-65)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PRTN3 |
| Synonyms | Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3 (PR-3; PR3); Neutrophil proteinase 4 (NP-4); P29; Wegener autoantigen; MBN |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P24158 |
| Clone Number | S-4942-65 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HL-60, THP-1 |
| Predicted Reactivity | Or, Cz |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
PRTN3 (proteinase 3) is a serine protease expressed mainly in neutrophil precursor cells, also known as myeloblastin because of its originally discovered antiproliferative activity. The human PRTN3 gene is located at chromosome 19p13.3, clustered with the neutrophil elastase (ELANE) and azurocidin genes; its expression is largely restricted to the promyelocyte/myelocyte stage of bone marrow development and is regulated by G-CSF and the PU.1 transcription factor. In terms of cellular localization, PRTN3 is stored mainly in the azurophilic granules of neutrophils and is released upon stimulation of neutrophils by TNF, LPS, or fMLP; after release, it can anchor to the cell surface by binding to CD177. Functionally, PRTN3 degrades elastin, fibronectin, laminin, and type I, III, and IV collagen, and enhances endothelial barrier function by cleaving and activating PAR-2, participating in the regulation of neutrophil transendothelial migration. Clinically, PRTN3 is one of the principal autoantigens in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, and anti-PR3 antibodies (PR3-ANCA) are closely associated with the diagnosis, disease activity, and relapse risk of granulomatosis with polyangiitis. Recent studies have also found that serum PRTN3 levels can predict the prognosis of patients with metastatic colorectal cancer receiving first-line chemotherapy (especially bevacizumab treatment), with high PRTN3 levels associated with worse progression-free survival; the mechanism may involve PRTN3 promoting angiogenesis and attenuating the antiangiogenic effect of bevacizumab. In addition, PRTN3 has attracted attention in myeloid hematopoietic differentiation and acute myeloid leukemia, suggesting that its functions extend well beyond serving as an autoantigen in vasculitis.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PRTN3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
