PRSS35 Recombinant Rabbit mAb (S-3769-241)

Catalog Number: S0B6895 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	PRSS35
Synonyms	Inactive serine protease 35; C6orf158
Immunogen	Synthetic Peptide
Location	Secreted
Accession	Q8N3Z0
Clone Number	S-3769-241
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	PC-3, mouse liver, rat liver
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

Background

PRSS35, also known as serine protease 35 or neurosin, is a member of the S1 family of chymotrypsin-like serine proteases that is predominantly expressed in the central nervous system, particularly within neurons and oligodendrocytes. This enzyme plays a critical role in various physiological processes, including the regulation of myelin maintenance, synaptic plasticity, and the processing of extracellular matrix proteins such as myelin basic protein (MBP) and proteolipid protein (PLP). Research suggests that PRSS35 is involved in the turnover of myelin components, thereby influencing nerve conduction velocity and overall neural integrity, while its dysregulation or overexpression has been implicated in pathological conditions such multiple sclerosis, Alzheimer’s disease, and certain types of cancer, where it may contribute to tissue remodeling, inflammation, and tumor progression through the degradation of specific substrates and modulation of cell signaling pathways.

Picture

Western Blot

WB result of PRSS35 Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDa

WB result of PRSS35 Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDa

WB result of Recombinant Rabbit mAb
Primary antibody: PRSS35 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 60 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-PRSS35 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in PC-3 cells. Anti-PRSS35 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).