M: marker
Lane 1: 2μg PK is reduced and thermally denatured;
Lane 2: 2μg PK under reducing condition;
Lane 3: 2μg PK is non-reduced and thermally denatured;
Lanes 4: 2μg PK under non-reducing condition;
Proteinase K(Tag free)
Proteinase K(Tag free)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
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Product Details
Product Details
Product Specification
| Synonyms | Proteinase K |
| Expression System | PichiaPastoris |
| Molecular Weight | 72 kD |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl、1 mM CaCl2、50% Glycerol、pH 7.4 @ 25°C |
| Stability & Storage | Store at -25 ~ -15℃ for 1 years |
| Reference | [1] Kushnirov VV, Dergalev AA, Alexandrov AI. Proteinase K resistant cores of prions and amyloids. Prion. 2020 Dec;14(1):11-19. |
Background
Proteinase K is a powerful proteolytic enzyme with high specific activity and is a key reagent for DNA extraction. The enzyme is active in a wide pH range (4~12.5) and at high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA. In DNA extraction, the main function is to enzymatically dissociate the histones bound to nucleic acids, so that the DNA is free in the solution, and then extracted by different methods to remove impurities and collect the DNA. chelating agents such as EDTA or descaling agents such as SDS can not inactivate the enzyme.
Components
Storage Solution:20 mM Tris-HCl、1mM CaCl2、50% glycerol (pH 7.4 @ 25°C)
Protocol
Add the specified amount of Proteinase K storage solution according to the nucleic acid extraction instructions. Commonly used working concentrations are 50-100 µg/mL
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
One unit is defined as amount of enzyme required to hydrolyze casein to produce 1µmoL L-tyrosine per minute under defined conditions.
Picture
Picture
SDS-PAGE
