WB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: HeLa whole cell lysate 5 µg
Lane 2: Jurkat whole cell lysate 5 µg
Lane 3: JAR whole cell lysate 5 µg
Lane 4: Hep G2 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
Profilin1 Recombinant Rabbit mAb (S-869-13)
Profilin1 Recombinant Rabbit mAb (S-869-13)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Epididymis tissue protein Li 184a, Profilin-1, PFN1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P07737 |
| Clone Number | S-869-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000 |
Background
Profilin1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. Mutations in this gene may be a rare cause of amyotrophic lateral sclerosis, also called Lou Gehrig's disease.
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Western Blot
WB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: NIH/3T3 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
WB result of Profilin1 Rabbit mAb
Primary antibody: Profilin1 Rabbit mAb at 1/2000 dilution
Lane 1: C6 whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
