WB result of PRODH2 Recombinant Rabbit mAb
Primary antibody: PRODH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 41 kDa
PRODH2 Recombinant Rabbit mAb (S-5085-19)
PRODH2 Recombinant Rabbit mAb (S-5085-19)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PRODH2 |
| Synonyms | Hydroxyproline dehydrogenase; Kidney and liver proline oxidase 1; MmPOX1; Probable proline dehydrogenase 2; Probable proline oxidase 2; Proline oxidase-like protein (PO; Proline oxidase); Hypdh; Prodh2 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q8VCZ9 |
| Clone Number | S-5085-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | Mouse liver, mouse kidney, mouse testis, rat liver, rat kidney |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:800 | Ms, Rt |
| IF | 1:400 | Ms, Rt |
Background
PRODH2 (proline dehydrogenase 2, also known as hydroxyproline dehydrogenase/HYPDH) is a FAD-dependent metabolic enzyme located mainly on the inner mitochondrial membrane that catalyzes the first step of trans-4-hydroxy-L-proline catabolism, oxidizing it to Δ-1-pyrroline-3-hydroxy-5-carboxylate. Human PRODH2 is 536 amino acids long, with its catalytic core located between residues 157 and 515; it strongly prefers hydroxyproline over proline as a substrate and uses coenzyme Q10 rather than oxygen as the terminal electron acceptor in vivo, and is therefore fundamentally a dehydrogenase rather than an oxidase. The enzyme is expressed at highest levels in the kidney and liver, and its downstream product glyoxylate can lead to elevated oxalate and calcium oxalate kidney stone formation in individuals with oxalate metabolism disorders, so PRODH2 is regarded as a potential therapeutic target for primary hyperoxaluria. In terms of genetic disease, biallelic pathogenic variants in PRODH2 are the genetic basis of hydroxyprolinemia, an autosomal recessive benign biochemical phenotype; patients are usually asymptomatic, but because hydroxyproline is difficult to distinguish from isoleucine/leucine in tandem mass spectrometry, it often causes false-positive newborn screening for maple syrup urine disease. In addition, recent studies have found that PRODH2 overexpression can significantly enhance the in vivo antitumor efficacy of CAR-T cells, expanding the potential application value of this metabolic enzyme in immunotherapy engineering.
Picture
Picture
Western Blot
WB result of PRODH2 Recombinant Rabbit mAb
Primary antibody: PRODH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 41 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse liver. Anti-PRODH2 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PRODH2 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-PRODH2 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PRODH2 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse liver. Anti-PRODH2 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse testis. Anti-PRODH2 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti-PRODH2 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti-PRODH2 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
