WB result of PRKCQ Recombinant Rabbit mAb
Primary antibody: PRKCQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Low expression control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa
PRKCQ Recombinant Rabbit mAb (S-2511-11)
PRKCQ Recombinant Rabbit mAb (S-2511-11)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PRKCQ |
| Synonyms | Protein kinase C theta type; nPKC-theta; PRKCT |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q04759 |
| Clone Number | S-2511-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | Jurkat, K562, Ramos, mouse spleen, mouse thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Protein kinase C theta (PKC-θ), encoded by the PRKCQ gene on chromosome 10p15.1, is a calcium-independent, phospholipid-dependent serine/threonine kinase selectively expressed in hematopoietic cells—especially platelets and T lymphocytes—where it transduces T-cell receptor signals to activate NF-κB and AP-1, thereby orchestrating T-cell activation, survival, and distinct Th17-mediated immune responses, while also promoting oncogenic growth and anoikis resistance in triple-negative breast cancer cells, and its kinase activity is considered a potential therapeutic target in autoimmunity and cancer.
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Picture
Western Blot
WB result of PRKCQ Recombinant Rabbit mAb
Primary antibody: PRKCQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa
