WB result of PRAME Recombinant Rabbit mAb
Primary antibody: PRAME Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A375 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: SK-MEL-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 52 kDa
PRAME Recombinant Rabbit mAb (S-1876-173)
PRAME Recombinant Rabbit mAb (S-1876-173)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PRAME |
| Synonyms | Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4 (OIP-4); Preferentially expressed antigen of melanoma; MAPE; OIP4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane, Cytoplasm, Nucleus |
| Accession | P78395 |
| Clone Number | S-1876-173 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A375, U-2 OS, K562, SK-MEL-2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
PRAME (Preferentially Expressed Antigen of Melanoma) is a protein encoded by the PRAME gene, belonging to the cancer-testis antigen family. It is predominantly expressed in various cancers, including melanoma, breast cancer, lung cancer, and leukemia, but is largely absent in normal tissues except for testis, ovary, placenta, and a few others. PRAME can inhibit retinoic acid signaling, thereby promoting cell proliferation and contributing to tumorigenesis. Due to its restricted expression pattern, PRAME is considered a promising target for cancer immunotherapy, with ongoing research exploring PRAME-specific vaccines and cellular immunotherapies.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti- PRAME antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
