WB result of PPARA Recombinant Rabbit mAb
Primary antibody: PPARA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
PPARA Recombinant Rabbit mAb (S-3103-24)
PPARA Recombinant Rabbit mAb (S-3103-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PPARA |
| Synonyms | Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; NR1C1; PPAR |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q07869 |
| Clone Number | S-3103-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, HepG2, HEK-293, Jurkat, NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms |
Background
PPARA (peroxisome proliferator-activated receptor alpha) is a ligand-activated transcription factor of the nuclear hormone receptor family that acts as a master regulator of lipid metabolism, primarily in liver, heart, skeletal muscle and kidney; upon binding fatty acids, eicosanoids or hypolipidemic fibrates it heterodimerizes with RXR, translocates to the nucleus and binds PPREs to activate genes driving peroxisomal and mitochondrial β-oxidation, ketogenesis, gluconeogenesis and lipoprotein remodeling while also exerting anti-inflammatory and anti-ferroptotic effects through cross-talk with GPX4 and other pathways.
Picture
Picture
Western Blot
WB result of PPARA Recombinant Rabbit mAb
Primary antibody: PPARA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
