The POLQ-N activity was detected using ADP-Glo technology. The reaction was performed by incubating the POLQ-N protein, ATP and ssDNA at 25℃ for 40 min, then adding ADP-Glo reagent and reading RLU with BMG.
POLtheta Protein
POLtheta Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | PRKCQ, PRKCT, MGC126514, MGC141919, nPKC-theta, PKCθ protein kinase c theta |
| Accession | O75417-1 |
| Amino Acid Sequence | M1-M894 |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 101831.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 500 mM NaCl, 2 mM B-ME, 5% glycerol, pH 8.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg POLQ-N
2 3 μg POLQ-N
