The POLQ-C activity was detected using TR-FRET technology. The reaction was performed by incubating the POLQ-C protein, fluorescence-labeled dsDNA and dNTP (dCTP, dGTP, dTTP) at 25℃ for 40 min, then reading ratio 520/485 fluorescence signal with BMG.
POLtheta Protein
POLtheta Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | PRKCQ, PRKCT, MGC126514, MGC141919, nPKC-theta, PKCθ protein kinase c theta |
| Accession | O75417-1 |
| Amino Acid Sequence | S1819-V2590(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 88602.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 500 mM NaCl, 2 mM B-ME, 5% glycerol, pH8.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg POLQ-C
2 3 μg POLQ-C
