WB result of POLD1 Recombinant Rabbit mAb
Primary antibody: POLD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: K-562 whole cell lysate 20 μg
Lane 3: MCF7 whole cell lysate 20 μg
Lane 4: A549 whole cell lysate 20 μg
Lane 5: MDA-MB-231 whole cell lysate 20 μg
Lane 6: Jurkat whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
POLD1 Recombinant Rabbit mAb (S-6027)
POLD1 Recombinant Rabbit mAb (S-6027)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | POLD1 |
| Location | Nucleus |
| Accession | P28340 |
| Clone Number | S-6027 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, K-562, MCF7, A549, MDA-MB-231, RAW264.7, NIH/3T3, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
| ICC | 1:100 | Hu |
Background
POLD1 (DNA polymerase delta catalytic subunit 1) is the large catalytic subunit p125 of DNA polymerase δ (Polδ), encoded by the POLD1 gene in the q13.3 region of human chromosome 19; it harbors DNA polymerase activity and 3′→5′ exonuclease (proofreading) activity and, together with the three small subunits POLD2, POLD3, and POLD4, forms the Polδ holoenzyme, which is mainly responsible for lagging-strand synthesis during DNA replication and also participates in multiple DNA repair pathways, including nucleotide excision repair, base excision repair, mismatch repair, and double-strand break repair. The exonuclease domain of POLD1 acts as a "proofreader," excising misincorporated nucleotides during replication to safeguard replication fidelity; germline pathogenic variants in this domain (such as L474P and S478N) markedly increase the mutation rate and cause autosomal dominant polymerase proofreading-associated polyposis (PPAP), predisposing carriers to colorectal and endometrial cancer. On the other hand, in-frame deletions in the polymerase active site of POLD1 (such as Ser605del) cause MDPL syndrome, characterized by mandibular hypoplasia, deafness, progeroid features, and lipodystrophy, with the mechanism involving the mutant protein gaining a motif that binds aberrantly to the telomere protein TRF1, linking POLD1 function to telomere biology and progeroid phenotypes. In addition, variants that reduce but do not completely abolish POLD1 polymerase activity can also cause non-syndromic sensorineural hearing loss.
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Western Blot
WB result of POLD1 Recombinant Rabbit mAb
Primary antibody: POLD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 μg
Lane 2: NIH/3T3 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
WB result of POLD1 Recombinant Rabbit mAb
Primary antibody: POLD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-POLD1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-POLD1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-POLD1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-POLD1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-POLD1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-POLD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-POLD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-POLD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-POLD1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
