IF shows positive staining in paraffin-embedded human gastric cancer. Anti-Podoplanin (D2-40) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Podoplanin (D2-40) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-1178-8)
Podoplanin (D2-40) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-1178-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Podoplanin (D2-40) |
| Synonyms | Aggrus, Glycoprotein 36 (Gp36), PA2.26 antigen, T1-alpha (T1A), PDPN, GP36 |
| Immunogen | Synthetic Peptide |
| Location | Membrane, Cell projection |
| Accession | Q86YL7 |
| Clone Number | SDT-1178-8 |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Positive Sample | Human tonsil, Human gastric cancer |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:50-1:100 | Hu |
Background
Podoplanin is a 40 kDa, transmembrane, oncofetal, O linked sialoglycoprotein (mucin type) found on lymphatic endothelium, mesothelium and fetal testis, , but not in microvascular endothelial cells. Podoplanin is selectively expressed in normal and malignant lymphatic endothelium, including Kaposi's sarcomas. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas and seminomas.
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-Podoplanin (D2-40) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
