PNPLA2 Recombinant Rabbit mAb (S-5409)

Catalog Number: S0B60414 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	PNPLA2
Synonyms	Patatin-like phospholipase domain-containing protein 2; Adipose triglyceride lipase; Calcium-independent phospholipase A2-zeta (iPLA2-zeta); Desnutrin; Pigment epithelium-derived factor receptor (PEDF-R); TTS2.2; Transport-secretion protein 2 (TTS2)
Location	Cytoplasm, Cell membrane
Accession	Q96AD5
Clone Number	S-5409
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	PANC-1, A549, HepG2, LNCaP, 3T3-L1, differentiated 3T3-L1, mouse adipose, rat adipose
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

PNPLA2 also known as adipose triglyceride lipase (ATGL), is the rate-limiting enzyme that initiates the first step of intracellular triglyceride hydrolysis, playing a central role in regulating systemic energy homeostasis. This protein consists of 504 amino acids, with a conserved patatin domain and a catalytic dyad composed of serine and aspartate at its N-terminus, while the C-terminus mediates its localization to the surface of lipid droplets, enabling efficient hydrolysis of triglycerides stored in the lipid droplet core. PNPLA2 is predominantly highly expressed in adipose tissue, with significant distribution also found in heart, skeletal muscle, and testis. Its activity is subject to intricate regulation: CGI-58 protein serves as its co-activator, enhancing its hydrolytic activity by approximately 20-fold, while proteins such as G0S2 and HILPDA inhibit its function through direct binding to ATGL. Dysfunction of this protein is closely associated with human diseases; inactivating mutations in its encoding gene can lead to neutral lipid storage disease with myopathy (NLSDM), characterized by abnormal accumulation of neutral lipids in multiple tissues including cardiac and skeletal muscle, often accompanied by severe skeletal myopathy and cardiomyopathy. Furthermore, as ATGL drives pathogenic cytosolic lipolysis in adipocytes and releases excess free fatty acids, its hyperfunction is associated with the development and progression of metabolic diseases such as obesity-related dyslipidemia, adipose tissue inflammation, and cardiac lipotoxicity, making it an important potential therapeutic target for cardiometabolic diseases.

Picture

Western Blot

WB result of PNPLA2 Recombinant Rabbit mAb
Primary antibody: PNPLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate

WB result of PNPLA2 Recombinant Rabbit mAb
Primary antibody: PNPLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa

WB result of PNPLA2 Recombinant Rabbit mAb
Primary antibody: PNPLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 3T3-L1 whole cell lysate 20 µg
Lane 2: differentiated 3T3-L1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate

WB result of PNPLA2 Recombinant Rabbit mAb
Primary antibody: PNPLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse adipose lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa

WB result of PNPLA2 Recombinant Rabbit mAb
Primary antibody: PNPLA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat adipose lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human adipose. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse white adipose. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brown adipose. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse testis. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat white adipose. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brown adipose. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat brain. Anti-PNPLA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.