WB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDa
PLK1 Recombinant Rabbit mAb (S-2835-32)
PLK1 Recombinant Rabbit mAb (S-2835-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PLK1 |
| Synonyms | Serine/threonine-protein kinase PLK1; Polo-like kinase 1 (PLK-1); Serine/threonine-protein kinase 13 (STPK13); PLK |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P53350 |
| Clone Number | S-2835-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Ramos, HepG2, HeLa, HEK-293, Jurkat, mouse testis, PC-12, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
PLK1 (Polo-like kinase 1) is a 603-residue serine/threonine kinase that peaks in late G2/M where it docks to phosphorylated Bub1 and the PBD-binding p-T78 motif on its own T-loop to auto-activate, then successively phosphorylates Cdc25C, Cyclin B1, Wee1, APC/C-Cdc20, and myriad kinetochore proteins to drive centrosome maturation, bipolar spindle assembly, chromosome alignment, the spindle-assembly and decatenation checkpoints, and timely mitotic exit, while its deregulated overexpression in cancers creates chromosomal instability and chemoresistance, making it a high-priority drug target whose ATP-competitive inhibitors (BI 2536, volasertib) are already in oncology trials.
Picture
Picture
Western Blot
WB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDa
WB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDa
WB result of PLK1 Recombinant Rabbit mAb
Primary antibody: PLK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 65 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-PLK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
