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Plectin His Tag Protein, Rhesus macaque/Cynomolgus

Plectin His Tag Protein, Rhesus macaque/Cynomolgus

Catalog Number: UA016101 Brand: UA BIOSCIENCE
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$235 USD
$235 USD
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Product Details

Product Specification


Species Cynomolgus, Rhesus macaque
Synonyms PCN, PLTN, Plectin-1, PLEC1
Accession Rhesus macaque :F7ABN9、Cynomolgus: XP_065375884.1
Amino Acid Sequence

Rhesus macaque :Ala4555-Ala4862 with His Tag at the N-Terminus
Cynomolgus:Ala4394-Ala4701 with His Tag at the N-Terminus
Expression System HEK293
Molecular Weight

40-60kDa (Reducing)
Purity ＞90% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Natsuga K, Nishie W, Akiyama M, Nakamura H, Shinkuma S, McMillan JR, Nagasaki A, Has C, Ouchi T, Ishiko A, Hirako Y, Owaribe K, Sawamura D, Bruckner-Tuderman L, Shimizu H. Plectin expression patterns determine two distinct subtypes of epidermolysis bullosa simplex. Hum Mutat. 2010 Mar;31(3):308-16.
2.Tu WT, Chen PC, Hou PC, Huang HY, Wang JY, Chao SC, Lee JY, McGrath JA, Natsuga K, Hsu CK. Plectin Missense Mutation p.Leu319Pro in the Pathogenesis of Autosomal Recessive Epidermolysis Bullosa Simplex. Acta Derm Venereol. 2020 Aug 18;100(15):adv00242.

Background

Plectin is a large cytolinker protein composed of an N-terminal actin-binding domain, a central rod-shaped α-helical coiled-coil region, and a C-terminal intermediate filament-binding domain, with multiple alternatively spliced isoforms enabling diverse subcellular localization; its functional core lies in bridging microfilaments, intermediate filaments, and microtubule networks to maintain cellular mechanical stability, while also participating in hemidesmosome assembly, myofibril integrity maintenance, and signal transduction regulation. Clinically, PLEC gene mutations cause epidermolysis bullosa simplex (EBS) and its variant subtypes with muscular dystrophy, pyloric atresia, or cardiomyopathy, collectively termed "plectinopathies," with pathological mechanisms involving dermal-epidermal junction disruption, desmin aggregation in muscle fibers, and mitochondrial dysfunction, while recent therapeutic strategies such as chemical chaperone approaches have provided new directions for treatment.

Picture

SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).