The activity of PKCα is verified by the ADP-Glo method: Incubate PKCα protein, ATP, and the
substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the
luminescence signal using the chemiluminescence module of the microplate reader.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P17252 |
Amino Acid Sequence | A2-V672 end,with N-GST-TEV |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 103.2 KDa |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose |
Stability & Storage | -80℃ |
Guidelines
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
Picture
Picture
Bioactivity
SDS-PAGE
