WB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 80 kDa
PKC beta Recombinant Rabbit mAb (S-3158-28)
PKC beta Recombinant Rabbit mAb (S-3158-28)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PKC beta |
| Synonyms | Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P05771 |
| Clone Number | S-3158-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, THP-1, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Protein kinase C beta (PKCβ) is a serine/threonine kinase of the conventional PKC subfamily that is activated by diacylglycerol and calcium; it phosphorylates diverse substrates to regulate glucose transport, vascular permeability, immune cell signaling and cell survival, and its sustained overactivity drives diabetic microvascular complications, atherosclerosis and certain cancers, making selective PKCβ inhibitors attractive therapeutic candidates.
Picture
Picture
Western Blot
WB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 90 kDa
WB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 80 kDa
Immunocytochemistry
ICC shows positive staining in K562 cells (top panel) and negative staining in HCT116 cells (below panel). Anti-PKC beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
