WB result of PIN1 Recombinant Rabbit mAb
Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
PIN1 Recombinant Rabbit mAb (S-2731-43)
PIN1 Recombinant Rabbit mAb (S-2731-43)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PIN1 |
| Synonyms | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1 (PPIase Pin1); Rotamase Pin1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13526 |
| Clone Number | S-2731-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, Jurkat, A549, HeLa, NIH/3T3, mouse heart, PC-12, rat brain, COS-7 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
PIN1 is a conserved 163-residue peptidyl-prolyl cis-trans isomerase composed of an N-terminal WW domain and a C-terminal catalytic PPIase domain, both recognizing pSer/Thr-Pro motifs; by selectively isomerizing these phosphorylated bonds it acts as a post-phosphorylation switch that reshapes the conformation, stability, localization and ubiquitination of hundreds of client proteins—ranging from p53 and cyclins to NF-κB and APP—thereby governing cell-cycle progression, transcription, immune signaling and neuronal homeostasis, while its frequent over-activation in cancer drives oncogenic pathways and its loss contributes to neurodegenerative diseases such as Alzheimer’s.
Picture
Picture
Western Blot
WB result of PIN1 Recombinant Rabbit mAb
Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
WB result of PIN1 Recombinant Rabbit mAb
Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
WB result of PIN1 Recombinant Rabbit mAb
Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
Immunocytochemistry
ICC shows positive staining in HEK293 cells. Anti- PIN1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
