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PIK3CA/PIK3R1 GST&Flag Tag Protein,Human

PIK3CA/PIK3R1 GST&Flag Tag Protein,Human

Catalog Number: UA080533 Brand: UA BIOSCIENCE
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$720 USD
$720 USD
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Product Details

Product Specification


Species Human
Synonyms PIK3CA,CLAPO,CLOVE,CWS5,MCAP,MCM,MCMTC,PI3K,PI3K-alpha,p110-alpha
Accession PIK3CA: P42336、PIK3R1: P27986-1
Amino Acid Sequence

PIK3CA: M1-N1068(end); N-terminal GST tag
PIK3R1: M1-R724(end); N-terminal Flag tag
Expression System Baculovirus-InsectCells
Molecular Weight PIK3CA: 150.9 kDa; PIK3R1: 84.9 kDa
Purity > 80% by SDS-PAGE
Conjugation Unconjugated
Tag GST Tag, Flag Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

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Bioactivity

The PIK3CA/PIK3R1 activity was activity was quantified using the ADP-Glo assay. The reaction was performed by incubating the PIK3CA/PIK3R1 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.

SDS-PAGE