WB result of PICK1 Recombinant Rabbit mAb
Primary antibody: PICK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
PICK1 Recombinant Rabbit mAb (S-2346-165)
PICK1 Recombinant Rabbit mAb (S-2346-165)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PICK1 |
| Synonyms | PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein; PRKCABP |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Synapse |
| Accession | Q9NRD5 |
| Clone Number | S-2346-165 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, NIH/3T3, C6, COS-7, C2C12, mouse brain, rat brain |
| Predicted Reactivity | Bv, Or |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Protein Interacting with C Kinase 1 (PICK1) is a 416-residue peripheral membrane scaffolding protein encoded by the PICK1 gene, comprising an N-terminal PDZ domain that binds the C-termini of membrane proteins such as AMPA-receptor subunits GluA2/3, dopamine transporter and acid-sensing ion channels, a central BAR domain that senses and induces membrane curvature, and a C-terminal acidic region; through PDZ-mediated interactions it clusters and traffics glutamate receptors to and from synapses, thereby regulating excitatory synaptic strength and plasticity underlying learning and memory, while its BAR domain cooperates with actin and endocytic machinery to control clathrin-mediated endocytosis, synaptic-vesicle recycling and dense-core vesicle biogenesis, and disruption of PICK1 in mice leads to impaired long-term depression, male infertility and neurotransmission deficits .
Picture
Picture
Western Blot
WB result of PICK1 Recombinant Rabbit mAb
Primary antibody: PICK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
WB result of PICK1 Recombinant Rabbit mAb
Primary antibody: PICK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
