WB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDa
PI3 Kinase p110β Recombinant Rabbit mAb (S-1193-115)
PI3 Kinase p110β Recombinant Rabbit mAb (S-1193-115)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PI3 Kinase p110 β |
| Synonyms | PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PIK3CB; PIK3C1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42338 |
| Clone Number | S-1193-115 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, MCF7, HeLa, C2C12, mouse brain, C6, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:50 | Hu, Ms |
Background
PI3 Kinase p110β, also known as PIK3CB, is a catalytic subunit of the phosphoinositide 3-kinase (PI3K) family, which plays a crucial role in cellular processes such as growth, metabolism, and tumorigenesis. This isoform, along with p110α, generates lipid second messengers that initiate multiple signal transduction cascades upon receptor activation. In the context of non-small cell lung cancer (NSCLC), p110β overexpression is associated with clinical significance and specific mechanisms. High expression rates of p110β in NSCLC have been reported to be around 41.8%. Its overexpression is particularly linked to the loss of PTEN, a negative regulator of the PI3K pathway, and is negatively correlated with EGFR mutations. This suggests that p110β may play a role in tumors where PTEN is deficient, potentially driving tumor survival and progression. Moreover, studies have shown that p110β has kinase-independent functions in regulating cell proliferation and trafficking.
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Western Blot
WB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDa
WB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p110β antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C2C12 cells. Anti- PI3 Kinase p110β antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
