WB result of PI3 Kinase p110 delta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
PI3 Kinase p110 delta Recombinant Rabbit mAb (S-M0023)
PI3 Kinase p110 delta Recombinant Rabbit mAb (S-M0023)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PIK3CD |
| Synonyms | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta (PtdIns-3-kinase subunit p110-delta; p110delta) |
| Location | Cytoplasm |
| Accession | O00329 |
| Clone Number | S-M0023 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, Jurkat, RAW264.7, mouse thymus, rat spleen |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
Background
PIK3CD is a gene that encodes a protein which is an integral part of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. This pathway is crucial for various cellular functions including cell growth, proliferation, survival, and metabolism. The PIK3CD protein specifically refers to the delta isoform of the catalytic subunit of PI3K. It is predominantly expressed in immune cells and plays a significant role in the regulation of immune responses. Abnormal activation or mutations in the PIK3CD gene can lead to a range of diseases including certain types of leukemia and other immune-related disorders. Understanding its function and regulation is important for developing targeted therapies in cancer and immune system disorders.
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Western Blot
WB result of PI3 Kinase p110 delta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
WB result of PI3 Kinase p110 delta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 delta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
