Phosphotyrosine Rabbit mAb (S-R207)

Catalog Number: S0B0319 Application: WB, IHC-P, IP Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$110 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	Phospho-Tyrosine
Synonyms	p‑Tyr, pY, 酪氨酸磷酸化
Clone Number	S-R207
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
Dot Blot	1:500
WB	1:1000
IP	1:50
IHC	1:500

Background

Tyrosine phosphorylation is the addition of a phosphate (PO43−) group to the amino acid tyrosine on a protein. It is one of the main types of protein phosphorylation. This transfer is made possible through enzymes called tyrosine kinases. Tyrosine phosphorylation is a key step in signal transduction and the regulation of enzymatic activity.

Picture

Western Blot

WB result of Phosphotyrosine Rabbit mAb
Primary antibody: Phosphotyrosine Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with Sodium pervanadate (3 mM, 30 min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: multiple
Observed MW: multiple
Exposure time: 180 s

IP

Phosphotyrosine Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Tyrosine in 0.4 mg HeLa treated with Sodium pervanadate (3 mM, 30 min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Tyrosine Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate 20 µg (Input)
Lane 2: Phospho-Tyrosine Rabbit mAb IP in HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate
Predicted MW: multiple
Observed MW: multiple
Exposure time: 30 s

Dot Blot

Dot blot result of Phosphotyrosine Rabbit mAb
Lane 1: Modified peptide library (Phospho-Serine)
Lane 2: Modified peptide library (Phospho-Threonine)
Lane 3: Modified peptide library (Phospho-Tyrosine)
Lane 4: Unmodified peptide library (Serine)
Lane 5: Unmodified peptide library (Threonine)
Lane 6: Unmodified peptide library (Tyrosine)
Primary antibody: Phosphotyrosine Rabbit mAb at 1/500 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse lung (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat lung (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.