Phosphothreonine Recombinant Rabbit mAb (S-4181-29)

Catalog Number: S0B60451 Application: WB, ICC Brand: Starter

Price:

$135 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Phosphothreonine
Synonyms	磷酸苏氨酸，苏氨酸磷酸化，p-Thr，pT
Immunogen	Synthetic Peptide
Clone Number	S-4181-29
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, ICC
Purification	Protein A
Concentration	1 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
Dot Blot	1:1000	Species Independent
WB	1:1000	Species Independent
ICC	1:100	Species Independent

Background

Phosphothreonine (pThr) is the phosphorylated ester of threonine and an important phosphoamino acid. It serves as a key participant in reversible protein phosphorylation—a core cellular signaling mechanism—and plays vital roles in both eukaryotes and prokaryotes. Similar to phosphorylation modification, its function is primarily achieved through two means: first, as a molecular "switch" that induces conformational changes in the modified protein to alter its activity; and second, as a "Lego block" that serves as a recognition site for specific structural domains (such as FHA domains), thereby mediating protein–protein interactions and assembling signaling complexes. Interestingly, structural studies have shown that phosphothreonine induces a more pronounced disorder-to-order transition in proteins compared to phosphoserine, and preferentially adopts a compact cyclic conformation, making its conformational changes more like a "step function" rather than the "rheostat"-like fine-tuning induced by phosphoserine. In living organisms, free phosphothreonine is scarce, but as an important post-translational modification form of proteins, it remains a core component in the regulatory networks governing numerous life processes.

Picture

Western Blot

WB result of Phosphothreonine Recombinant Rabbit mAb
Primary antibody: Phosphothreonine Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100ng/ml Calyculin A for 30mintes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

WB result of Phosphothreonine Recombinant Rabbit mAb
Primary antibody: Phosphothreonine Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100ng/ml Calyculin A for 30mintes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

WB result of Phosphothreonine Recombinant Rabbit mAb
Primary antibody: Phosphothreonine Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100ng/ml Calyculin A for 30mintes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple

Dot Blot

Dot blot result of Phosphothreonine Recombinant Rabbit mAb
Lane 1: Modified peptide library (Phospho-Serine)
Lane 2: Modified peptide library (Phospho-Threonine)
Lane 3: Modified peptide library (Phospho-Tyrosine)
Lane 4: Unmodified peptide library (Serine)
Lane 5: Unmodified peptide library (Threonine)
Lane 6: Unmodified peptide library (Tyrosine)
Primary antibody: Phosphothreonine Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Immunocytochemistry

ICC analysis of HeLa cells treated with Calyculin A (100nM/ml, 30min) (top panel) and untreated HeLa cells (below panel). Anti-Phosphothreonine antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).