WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
Phospho-YAP (Ser127) Recombinant Rabbit mAb (S-4250)
Phospho-YAP (Ser127) Recombinant Rabbit mAb (S-4250)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-YAP (Ser127) |
| Synonyms | Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP65; YAP1 |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P46937 |
| Clone Number | S-4250 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, PANC-1, Calyculin A treated HeLa, NIH/3T3, C2C12, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
Phospho-YAP (Ser127) is a phosphorylated form of the Yes-associated protein (YAP), which is a key transcriptional coactivator in the Hippo signaling pathway. YAP regulates various cellular processes, including cell proliferation, survival, differentiation, and organ size. The phosphorylation of YAP at serine 127 by kinases such as MST1/2 and LATS1/2 leads to its inactivation and cytoplasmic retention. When dephosphorylated, YAP can translocate to the nucleus and promote gene transcription. For example, androgen exposure can suppress the inhibitory phospho-Ser127 modification on YAP, resulting in increased YAP nuclear abundance and activity. This regulation is crucial for understanding the role of YAP in diseases like cancer.
Picture
Picture
Western Blot
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
This blot was developed with high sensitivity substrate
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung cancer. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
