WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
Phospho-YAP (Ser127) Recombinant Rabbit mAb (S-1364-28)
Phospho-YAP (Ser127) Recombinant Rabbit mAb (S-1364-28)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-YAP (Ser127) |
| Synonyms | Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP65; YAP1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P46937 |
| Clone Number | S-1364-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Predicted Reactivity | Ck, Zf |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu, Ms |
Background
Phospho-YAP (Ser127) is a phosphorylated form of the Yes-associated protein (YAP), which is a key transcriptional coactivator in the Hippo signaling pathway. YAP regulates various cellular processes, including cell proliferation, survival, differentiation, and organ size. The phosphorylation of YAP at serine 127 by kinases such as MST1/2 and LATS1/2 leads to its inactivation and cytoplasmic retention. When dephosphorylated, YAP can translocate to the nucleus and promote gene transcription. For example, androgen exposure can suppress the inhibitory phospho-Ser127 modification on YAP, resulting in increased YAP nuclear abundance and activity. This regulation is crucial for understanding the role of YAP in diseases like cancer.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of HeLa cells treated with Calyculin A (100ng/ml,30min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of NIH/3T3 cells treated with Calyculin A (100nM, 30min) (top panel) and untreated NIH/3T3 cells (below panel). Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
