WB result of Phospho-Vimentin (Ser56) Recombinant Rabbit mAb
Primary antibody: Phospho-Vimentin (Ser56) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Paclitaxel for 20 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 57 kDa
Phospho-Vimentin (Ser56) Recombinant Rabbit mAb (S-2135-5)
Phospho-Vimentin (Ser56) Recombinant Rabbit mAb (S-2135-5)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Vimentin (Ser56) |
| Synonyms | Vimentin; VIM |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Cell membrane |
| Accession | P08670 |
| Clone Number | S-2135-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Predicted Reactivity | AfGrMk, Cz, Pg, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Background
Phospho-Vimentin (Ser56) is a phosphorylated form of the vimentin protein, specifically at serine residue 56. Vimentin is a type of intermediate filament protein that plays a crucial role in maintaining cell integrity and providing mechanical support to cells. When phosphorylated at Ser56, it often indicates involvement in cellular processes such as cell migration, adhesion, and the epithelial-mesenchymal transition (EMT). This phosphorylation can be a key marker in various biological contexts, including cancer progression, where it may be associated with the enhanced motility and invasive potential of tumor cells.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-Vimentin (Ser56) Recombinant Rabbit mAb
Lane 1: Vimentin (Ser56) phospho peptide
Lane 2: Vimentin unmodified peptide
Primary antibody: Phospho-Vimentin (Ser56) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
