WB result of Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 starved for 4 hours, then treated with 100 ng/ml β-NGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 150 kDa
This blot was developed with high sensitivity substrate
Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb (S-3812)
Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb (S-3812)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-TrkA (Tyr490)/TrkB (Tyr516) |
| Synonyms | High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type; Tyrosine kinase receptor; TRK |
| Location | Endosome, Cell membrane |
| Accession | P04629、Q16620 |
| Clone Number | S-3812 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Rt |
| Positive Sample | β-NGF treated PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Rt |
Background
Phospho-TrkA (Tyr490) and phospho-TrkB (Tyr516) are the neurotrophin-activated, autophosphorylated forms of the receptor tyrosine kinases TrkA and TrkB; once these juxtamembrane tyrosines are phosphorylated they serve as docking platforms for adaptor proteins—TrkA-pTyr490 recruits FRS2 and Shc to trigger Ras/MAPK and PI3K/Akt cascades that mediate neuronal survival and differentiation, whereas TrkB-pTyr516 binds Shc to activate the same pathways plus PLCγ-driven PKC and Ca²⁺ signals that regulate synaptic plasticity—so antibodies detecting these phospho-sites are widely used as biomarkers for NGF- or BDNF-dependent Trk signaling in health and disease.
Picture
Picture
Western Blot
