WB result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb (S-1896-21)
Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb (S-1896-21)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-TGF β Receptor I(Ser165) |
| Synonyms | TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5 (ALK-5; ALK5); Serine/threonine-protein kinase receptor R4 (SKR4); TGF-beta type I receptor; Transforming growth factor-beta receptor type I (TGF-beta receptor type I; TbetaR-I); ALK5; SKR4; TGFBR1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P36897 |
| Clone Number | S-1896-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa treated with 100 ng/ml Calyculin A for 30 minutes, Serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Phospho-TGF β Receptor I (Ser165) is a phosphorylated form of the Transforming Growth Factor-beta Receptor Type I (TGFBR1), a transmembrane serine/threonine kinase that plays a crucial role in transmitting TGF-beta signals from the cell surface to the cytoplasm. This receptor forms a complex with TGF-beta type II receptors and is activated upon binding to TGF-beta ligands, leading to the phosphorylation of downstream SMAD proteins, which then regulate gene transcription involved in cell cycle control, differentiation, wound healing, and immune responses. The phosphorylation at Ser165 is particularly important for its activation and signaling, and this site is often targeted by antibodies for research applications such as Western blotting, immunohistochemistry, and immunofluorescence.
Picture
Picture
Western Blot
WB result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
Dot Blot
Dot blot result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
Lane 1: TGF β Receptor I (Ser165) phospho peptide
Lane 2: TGF β Receptor I (Ser165) unmodified peptide
Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of HeLa cells treated with 100 ng/ml Calyculin A for 30 minutes (top panel) and untreated HeLa cells (below panel). Anti- Phospho-TGF β Receptor I(Ser165) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
