WB result of Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb
Primary antibody: Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with serum starvation for overnight, add 100nM Calyculin A for 30 min whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 84 kDa
Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb (S-5532)
Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb (S-5532)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-NAK/TBK1 (Ser172) |
| Synonyms | Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; NAK; TBK1 |
| Location | Cytoplasm |
| Accession | Q9UHD2 |
| Clone Number | S-5532 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
Background
Phospho-NAK/TBK1 (Ser172) protein is a crucial component in cellular signaling pathways. NAK, also known as TBK1, is a serine/threonine kinase that plays a significant role in innate immune responses and NF-κB activation. When phosphorylated at serine 172, it becomes activated and can phosphorylate downstream substrates such as IRF3, leading to the production of type I interferons. This phosphorylation event at Ser172 is essential for its kinase activity and its involvement in antiviral signaling and inflammatory responses. Additionally, TBK1 has been implicated in autophagy regulation through its interaction with other proteins, further highlighting its multifaceted functions in maintaining cellular homeostasis and responding to various stressors.
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Western Blot
WB result of Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb
Primary antibody: Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: untreated RAW 264.7 whole cell lysate 20 µg
Lane 2: RAW 264.7 treated with 100nM Calyculin A for 30 min whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 84 kDa
WB result of Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb
Primary antibody: Phospho-TBK1/NAK (Ser172) Recombinant Rabbit mAb at 1/1000 dilution overnight at 4°C
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with serum starvation for overnight, add 100nM Calyculin A for 30 min whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 84 kDa
