WB result of Phospho-STING (Ser366) Recombinant Rabbit mAb
Primary antibody: Phospho-STING (Ser366) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 differentiated with 80 nM TPA for 16 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate
Phospho-STING (Ser366) Recombinant Rabbit mAb (S-2243-62)
Phospho-STING (Ser366) Recombinant Rabbit mAb (S-2243-62)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-STING (Ser366) |
| Synonyms | Stimulator of interferon genes protein; hSTING; Endoplasmic reticulum interferon stimulator (ERIS); Mediator of IRF3 activation (hMITA); Transmembrane protein 173; ERIS; MITA s; STING s; TMEM173; STING1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Lysosome, Mitochondrion, Cell membrane, Endoplasmic reticulum |
| Accession | Q86WV6 |
| Clone Number | S-2243-62 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:2000 | Hu |
Background
Phospho-STING (Ser366) protein is a phosphorylated form of the stimulator of interferon genes (STING) protein, specifically phosphorylated at serine 366. STING is a key component of the innate immune system, playing a crucial role in the cytosolic DNA sensing pathway. When phosphorylated at Ser366, STING is activated and promotes the production of type I interferons and other pro-inflammatory cytokines in response to pathogen-associated molecular patterns or DNA damage. This phosphorylation event is essential for the downstream signaling cascade that leads to the activation of immune cells and the initiation of an antiviral or antibacterial response. Abnormal regulation of Phospho-STING (Ser366) has been implicated in various immune-related diseases, making it a potential target for therapeutic interventions.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-STING (Ser366) Recombinant Rabbit mAb
Lane 1: STING (Ser366) phospho peptide
Lane 2: STING (Ser366) unmodified peptide
Primary antibody: Phospho-STING (Ser366) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
