WB result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 88 kDa
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360)
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Stat3 (Tyr705) |
| Synonyms | Signal transducer and activator of transcription 3; Acute-phase response factor; APRF |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P40763 |
| Clone Number | S-515-360 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Jurkat treated with IFN-α, HeLa treated with IFN-α |
| Predicted Reactivity | Pg, Cw |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
Background
Phospho-Stat3 (Tyr705) refers to the phosphorylated form of Signal Transducer and Activator of Transcription 3 (STAT3) at the tyrosine 705 residue. Phosphorylation of STAT3 at Tyr705 is a critical step in the activation of the STAT3 signaling pathway. This phosphorylation event, often induced by cytokines, growth factors, and oncogenes, leads to the formation of STAT3 dimers, which then translocate to the nucleus to initiate gene transcription. STAT3 is involved in various cellular processes, including cell growth, survival, and immune responses. Phospho-Stat3 (Tyr705) is essential for mediating cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. In the context of immunity, STAT3 plays a crucial role in regulating the differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg), which is controlled by the phosphorylation at Tyr705. Abnormal activation of STAT3, including constitutive phosphorylation at Tyr705, has been observed in various cancers and is associated with tumor growth, survival, and angiogenesis. In some cases, STAT3 activation is driven by autocrine secretion of interleukin-6 (IL-6), leading to continuous activation of the Jak/Stat pathway.
Picture
Picture
Western Blot
IP
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate 40 µg (Input)
Lane 2: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb IP in Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate
Predicted MW: 88 kDa
Observed MW: 88 kDa
Exposure time: 90 s
Dot Blot
Dot blot result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
Lane1: Stat3 (Tyr705) phospho peptide
Lane2: Stat3 unmodified peptide
Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
