WB result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 80, 90 kDa
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-1829-143)
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-1829-143)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Stat3 (Tyr705) |
| Synonyms | Signal transducer and activator of transcription 3; Acute-phase response factor; APRF; STAT3 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P40763 |
| Clone Number | S-1829-143 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
Background
Phospho-Stat3 (Tyr705) is a critical biomarker indicating the activation of the STAT3 protein, a latent cytoplasmic transcription factor involved in various cellular processes such as proliferation, survival, and immune response. When phosphorylated at tyrosine 705, STAT3 undergoes dimerization and translocates to the nucleus, where it binds to DNA and regulates gene expression. This phosphorylation is primarily mediated by the JAK family of receptor-associated tyrosine kinases, particularly JAK1, in response to cytokine signaling. Phospho-Stat3 (Tyr705) is also implicated in diseases like cancer, where its constitutive activation is observed in various tumor types.
Picture
Picture
Western Blot
IP
Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa+IFN-α(50 ng/ml, 30 min) whole cell lysate 20 µg (Input)
Lane 2: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb IP in HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate
Predicted MW: 88 kDa
Observed MW: 80, 90 kDa
Dot Blot
Dot blot result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
Lane 1: Stat3 (Tyr705) phospho peptide
Lane 2: Stat3 unmodified peptide
Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
