WB result of Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb
Primary antibody: Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa serum starvation overnight then treated with 10 ng/ml TGF-β3 for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 60 kDa
Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb (S-6222)
Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb (S-6222)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-SMAD2 (Ser465/467) |
| Synonyms | SMAD family member 2; SMAD 2 Curated; hSMAD2; JV18-1; Mad-related protein 2 (hMAD-2); Mothers against decapentaplegic homolog 2 (MAD homolog 2; Mothers against DPP homolog 2); MADH2; MADR2; SMAD2 |
| Location | Cytoplasm, Nucleus |
| Accession | Q15796 |
| Clone Number | S-6222 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Phospho-SMAD2 (Ser465/467) refers to the activated form of the SMAD2 protein, in which the TGF-β type I receptor kinase (TβRI) specifically phosphorylates two serine residues at positions 465 and 467 within the C-terminal SSXS motif. This phosphorylation event is the critical step in canonical TGF-β signaling: it enables SMAD2 to dissociate from the activated receptor complex and form a heterodimeric complex with the co-mediator SMAD4, which then translocates into the nucleus to regulate target gene transcription. Notably, phosphorylation at both sites is required for this function, as mutation of either serine 465 or 467 prevents SMAD2 release from the receptor and blocks TGF-β-dependent signaling. Additionally, phosphorylation at Ser465 is prerequisite for phosphorylation at Ser467, and these C-terminal phosphoserines serve as a recognition site for SMAD4 binding.
Picture
Picture
Western Blot
WB result of Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb
Primary antibody: Phospho-SMAD2 (Ser465/467) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 serum starvation overnight then treated with 10 ng/ml TGF-β3 for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 60 kDa
