WB result of Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 starve overnight, then grown in 20% FBS media for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb (S-4983)
Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb (S-4983)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-S6 Ribosomal Protein (Ser240/244) |
| Synonyms | Small ribosomal subunit protein eS6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6 |
| Location | Cytoplasm, Nucleus |
| Accession | P62753 |
| Clone Number | S-4983 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in HeLa cells treated with insulin (100 nM, 15 mins) (top panel) and negative staining in HeLa cells treated with LY294002 (50 μM, 2 hr) (below panel). Anti-Phospho-S6 Ribosomal Protein (Ser240/244 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
